These improvements are achieved by combining salt and pH gradients for preparative protein separations in combination with the development of a preparative step elution protein separation based on data generated by combined salt-pH gradient runs. Improved protein separation in ion exchange chromatography The present invention relates to improved preparative (>5 g/l) protein separations. C07K2317/52- Constant or Fc region Isotype.C07K2317/50- Immunoglobulins specific features characterized by immunoglobulin fragments.C07K2317/00- Immunoglobulins specific features. C07K16/065- Purification, fragmentation.monoclonal or polyclonal antibodies from serum C07K1/165- Extraction Separation Purification by chromatography mixed-mode chromatography.C07K1/16- Extraction Separation Purification by chromatography.C07K1/14- Extraction Separation Purification.processes for the organic chemical preparation of peptides or proteins of any length C07K1/00- General methods for the preparation of peptides, i.e.chromatography characterised by the separation mechanism involving ionic interaction chromatography characterised by the separation mechanism B01D15/00- Separating processes involving the treatment of liquids with solid sorbents Apparatus therefor.B01- PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL.Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Priority claimed from EP15195242.1 external-priority Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH Publication of CA3005483A1 publication Critical patent/CA3005483A1/en Status Abandoned legal-status Critical Current Links Original Assignee Merck Patent GmbH Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) Inventor Matthias Joehnck Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Abandoned Application number CA3005483A Other languages French ( fr) Google Patents Improved protein separation in ion exchange chromatographyÄownload PDF Info Publication number CA3005483A1 CA3005483A1 CA3005483A CA3005483A CA3005483A1 CA 3005483 A1 CA3005483 A1 CA 3005483A1 CA 3005483 A CA3005483 A CA 3005483A CA 3005483 A CA3005483 A CA 3005483A CA 3005483 A1 CA3005483 A1 CA 3005483A1 Authority CA Canada Prior art keywords gradient salt proteins buffer elution Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. Google Patents CA3005483A1 - Improved protein separation in ion exchange chromatography CA3005483A1 - Improved protein separation in ion exchange chromatography
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